Journal: Frontiers in Cardiovascular Medicine
Article Title: Heart rate reduction after genetic ablation of L-type Ca v 1.3 channels induces cardioprotection against ischemia-reperfusion injury
doi: 10.3389/fcvm.2023.1134503
Figure Lengend Snippet: ( A ) In vivo experimental protocol applied to wild-type (WT) mice, consisting of 40 min of ischemia induced by ligation of the left coronary artery followed by 60 min of reperfusion. Mice were assigned to receive either 0.9% NaCl (Ctrl, n = 6) or Ivabradine (Iva, 6 mg/kg, n = 7), intraperitoneally 30 min before ischemia. ECG was recorded during the surgical protocol; ( B ) scatter dot plots and mean ± SD were plotted for baseline HR measured before ischemia in control mice (NaCl 0.9%) ( n = 6) or Iva pre-treated mice ( n = 7); ( C ) scatter dot plots and mean ± SD were plotted for infarct size (expressed as the percentage of the area at risk) and measured at the end of the surgical protocol in control (Ctrl, n = 6) and Iva pre-treated (Iva, n = 7) mice subjected to ischemia-reperfusion (IR) protocol; Statistical analysis was performed using the non-parametric Mann–Whitney test. Statistical significance is noted ** for p = 0.0047 for ( B,C ).
Article Snippet: One-lead surface electrocardiograms (ECG) were recorded by Ag/AgCl gel-coated electrodes (Unomedical) attached to the superior right and both inferior limbs of mice and connected to a standard one-lead ECG amplifier module (EMKA Technologies, Paris, France).
Techniques: In Vivo, Ligation, Control, MANN-WHITNEY